QIAGEN QIAseq SPE Chemistry

QIAseq and Single Primer Extension Chemistry: Redefining Amplicon-Based NGS

Targeted NGS has been widely used for the detection and confirmation of genetic changes, yet there are technical challenges that undermine precise quantification and confound analyses. Multiplex PCR and nested PCR-based library preparation approaches present primer design and specificity challenges inherent to these methods. Amplicon-based next-generation sequencing (NGS) assays offer many advantages for targeted enrichment…. Read article →