High sequencing depth may increase the sensitivity of variant detection for bulk samples, but it has not proven appropriate for single cell sequencing. What’s more, it makes whole genome sequencing prohibitively expensive.
For variant detection in rare cells, such as circulating tumor cells, Zhang et al. recently presented a brilliant way to overcome these challenges: low depth sequencing of multiple single cells and census-based variant detection. For your convenience, we’ve summarized the concept in a new infographic.
Zhang, C.-Z. et al. (2015) Calibrating genomic and allelic coverage bias in single-cell sequencing, Nat. Comm. 6, 6822. (link)