Back to basics: a complete guide to qPCR and RT-qPCR technology


Are you interested in studying gene expression in an upcoming project but not sure where to begin? If so, you can sit back and relax, as we have made the slide presentations of our qPCR and RT-qPCR Technology Webinar Series available in SlideShare. This particular webinar series is one of the most popular ones in QIAGEN’s history. It begins with the basic steps of a qPCR experiment and covers the underlying technology as well as targeted qPCR arrays and data interpretation. Whether you want to refresh your knowledge on the fundamental concepts of real-time PCR – or whether you are trying to advance in your coding and non-coding RNA expression analysis studies – we have the resources to help you get started.

Part 1: Introduction to Real-Time PCR (Q-PCR/qPCR/qrt-PCR) 

This slidedeck provides the concepts of real-time PCR and introduces how to conduct a real-time PCR assay. You will receive an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors that will help support your research success.

Part 2: Advanced Real-Time PCR Array Technology – Coding and Noncoding RNA Expression Analysis 

Looking for a simple and accurate real-time PCR system for relevant biological pathway- and disease-focused mRNA and for long noncoding RNA (lncRNA) expression profiling? This slidedeck talks about the stringent performance built into the technology to ensure its sensitivity, specificity, reproducibility and reliability. You will learn how to use this technology in your research.

Part 3: PCR Array Data Analysis Tutorial 

With a step-by-step demonstration using actual PCR array data, this presentation introduces an easy-to-use and complimentary web-based data analysis tool to calculate fold differences in gene expression from your raw real-time PCR threshold cycles. Learn how you can look at your results in different formats – including heat maps, scatter and volcano plots, clustergrams and more. The data analysis tool also suggests further steps to expand upon and improve your research.


Part 4: Accelerate Your Discovery with QIAGEN’s Service Solutions for Biomarker Research

This slidedeck covers QIAGEN’s service capabilities in sample isolation, microarray, NGS-sequencing, qPCR panels, custom assay development and bioinformatics – all useful tools for identifying potential biomarkers and gene signatures. You will learn the applications of QIAGEN’s Service Core in microRNA discovery for toxicology markers in serum and plasma, and in the identification of RNA signatures for tumor stratification.

Bonus presentation: Building a Custom RT2 Profiler PCR Array in GeneGlobe

Interested in creating arrays and assays according to your particular research needs? Whether you are conducting mRNA expression, targeted NGS sequencing, ELISA protein analysis or simply the analysis of siRNA and miRNA mimics and inhibitors – in this slidedeck, we will show you how to best proceed.

Lily Xu Xuanyan

Lily Xu graduated from Leiden University, The Netherlands, after studying biochemistry and science-based business. With a background in both molecular genetics and the commercialization of new technologies, Lily joined QIAGEN in 2012. Since then, she has been involved in managing various parts of QIAGEN’s sample and assay portfolio. Lily's interests span several emerging fields, particularly single-cell analysis and microRNA biomarker discovery.

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