The world of next-generation sequencing (NGS) is both exciting and fast-moving. It offers an incredible amount of discovery and innovation, as well as a wide range of scientific applications. With many new NGS products recently developed at QIAGEN, we have added several educational NGS resources at SlideShare – from infographics to webinars and videos.
Next-generation sequencing, an introduction to technology and applications
This slide deck provides a technical overview of DNA/RNA preprocessing, template preparation, sequencing and data analysis. It also covers the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.
Next-generation sequencing for cancer research
DNA sequence information is important for virtually all branches of biomedical research. Given the genetic aspect of the disease, cancer research is at the forefront of genome sequencing. This slide deck discusses the most biologically efficient and cost-effective way to use NGS technology in cancer research. Here, you’ll be introduced to QIAGEN‘s DNAseq targeted enrichment system, which allows you to quickly and reliably sequence your genes of interest.
Next-generation sequencing data analysis for genetic profiling
Are you frustrated with millions of sequencing reads produced by NGS runs? Download this slide deck for help. It discusses the challenges of NGS data analysis – as well as the solutions provided by the QIAGEN NGS data analysis portal for identifying genetic variants from cell, tissue and FFPE samples. Using raw sequencing data, we demonstrate how our web-based data analysis portal can be used to discover both known and novel variants, and how to derive a biological meaningful interpretation from NGS data.
Meet the NGS experts series, Part 1: NGS in clinical research
In this slide deck, Dr. Birgit Funke (Associate Professor of Pathology at Harvard Medical School) gives an overview of state-of-the-art clinical NGS. She also discusses validation, clinical implementation and the migration from gene panels to exome sequencing for inherited disorders with clinical and genetic heterogeneity. In addition, important shortcomings such as difficulties with regions of high-sequence homology are discussed.
Meet the NGS experts series, Part 2: Translational genomics and prostate cancer
Dr. Johann de Bono (Professor at the Institute of Cancer Research and Royal Marsden NHS Foundation) presents an international research effort on prostate cancer in this SlideShare presentation. He and colleagues have dissected the molecular landscape of advanced castration-resistant prostate cancer, elucidating key molecular targets in this group of diseases. They have also shown that PARP inhibitors have anti-tumor activity against a significant proportion of these cancers – mainly in men whose cancers harbor DNA repair defects.
Meet the NGS experts series, Part 3: Utilization of NGS to identify clinically relevant mutations in cell-free circulating tumor DNA
Here, Dr. Clifford G. Tepper (Technical Director of the UC Davis Comprehensive Cancer Center’s Genomics Shared Resource, GSR) shares a recent study on pancreatic cancer using NGS. The study shows that KRAS mutations can be detected in cell-free, circulating tumor DNA (ctDNA) isolated from the plasma in a subset of patients – and that they are associated with poor prognosis. The ability to simultaneously detect multiple pancreatic cancer-specific mutations in ctDNA would open a new avenue for detection of clinically relevant mutations.
Meet the NGS experts series, Part 4: Developing a rapid clinical sequencing system to classify meningioma
Dr. Nishihara (Professor in the Department of Translational Pathology at Hokkaido University) shares his experience here of using targeted amplicon sequencing to develop a rapid clinical tool capable of identifying specific meningioma genotypes in just two weeks. The details of three meningioma categories and their genes are also discussed.
Infographic on NGS clinical research milestones
Interested in the history of the next-generation sequencing revolution? Check out our infographic on NGS clinical research milestones that traces the major clinical research events starting from 2001 to today!
The applications presented here are for research use only. Not for use in diagnostic procedures.
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