The quality of extracted genomic DNA (gDNA) is a critical factor in many downstream applications, such as real-time PCR or multiplex PCR.
Successful extraction of gDNA from formalin-fixed and paraffin-embedded (FFPE) tissues remains a challenge. Therefore, it’s good to get back to the basics of sample purification, multiplex PCR and quality control in order to better understand, address and resolve the challenges associated with every step.
We’re holding an exclusive 3-part webinar series starting Monday, March 06, to tackle the toughest questions in gDNA sample preparation and offer you tips for troubleshooting. Registration is free, and you only have to sign up once to be registered for all sessions. After the event, you’ll receive a link to view an on-demand recording.
Check out the details of all 3 sessions below:
Part 1: Fundamental concepts and special considerations in gDNA isolation for better insight – Available on demand
Speaker: Abhishek Sharma
How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. In this webinar, we will cover:
• The basic methods and challenges of gDNA purification
• Working with DNA: Good laboratory practice
• Special considerations for challenging sample types
• Performing DNA quality checks
Part 2: Nucleic acid quantification from FFPE samples; are you doing it right?
March 13, 1 p.m. EST, 6 p.m. CET
Speaker: Dr. Pierre-Henri Ferdinand
Formalin-fixation and paraffin-embedding is a standard method for long-term preservation of tissue biopsies. These stored samples are a valuable tool for studying diseases such as cancer, especially when they are histologically and pathologically well characterized, and follow-up clinical data is available. The quality of nucleic acids extracted from FFPE samples is influenced by a number of factors, including how the samples were handled before, during and after fixation and embedding. Moreover, there are several difficulties when purifying nucleic acids from FFPE samples as the chemicals and temperature used during the process can degrade the DNA.
In this webinar, we will:
• Discuss the variability in quantity and purity of DNA purified from FFPE material
• Show data from different quantification and quality control methods
• Demonstrate the impact of inaccurate quantification on downstream results and how to overcome these challenges
Part 3: Multiplex PCR for genotyping: Technology overview and applications
March 20, 1 p.m. EST, 6 p.m. CET
Speaker: Dr. Vishwadeepak Tripathi
Multiplex end-point PCR is a powerful tool for genotyping and many other applications. The technology itself has a number of advantages, but also has its own set of challenges. The key advantages and benefits of multiplex PCR are listed in the table below:
|Co-amplification of internal controls/reference genes||Increased reliability|
|Conservation of precious samples||More data per reaction|
|Increased throughput||More targets per time|
|Reduced reagent costs||More results per dollar|
To learn more about the critical factors that need to be considered for a successful multiplex end-point PCR, save your spot to join the live webinar and ask your questions during the Q&A session.
Sign up for free to hear more from our experts!