Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced.
Multiplex PCR has a multitude of advantages and applications. However, PCR multiplexing and analysis of resulting amplicons using conventional slab-gel electrophoresis present their own challenges.
Traditionally, multiplex PCR required extensive optimization procedures and use of conventional separation technologies (such as agarose slab gels) which often do not provide sufficient resolution for post-multiplex PCR analysis and are not always optimal for separation of amplicons that differ by just a few base pairs. In addition, the detection process is laborious, time-consuming and hazardous, due to manual pouring and running of gels while exposing yourself to compounds like ethidium bromide.
We’re holding a 2-part webinar series on April 20 and 27 to address these challenges and present innovative solutions that enable easy and sensitive multiplex PCR and superior DNA fragment analysis within 10 minutes. Registration is free, and you only have to sign up once to be registered for all sessions. After the event, you’ll receive a link to view an on-demand recording.
Check out the details of these 2 sessions below:
Part 1: Critical factors for successful multiplex PCR
April 20, 9:30 am EDT, 2:30 pm GMT, 3:30 pm CEST
Speaker: Dr. Vishwadeepak Tripathi
Multiplex end-point PCR is a powerful tool for genotyping and many other applications. QIAGEN’s multiplex PCR chemistry is optimized for reliable amplification of many different templates with high variability in copy numbers. Thus it enables very quick establishment of a new lab routine and assures instant success of your multiplex PCR strategy. Considering a set of critical factors will help you achieve the results you want, and these will be discussed in detail during the webinar. Additionally, our multiplex PCR chemistry has recently become popular among scientists who are performing next-generation sequencing. We will also briefly touch upon this topic.
Part 2: Increasing productivity in multiplex PCR applications with QIAxcel
April 27, 9:30 am EDT, 2:30 pm GMT, 3:30 pm CEST
Speaker: Dr. Pierre-Henri Ferdinand
The most commonly used method for analysis of genotyping assays based on end-point PCR is gel electrophoresis, using manually poured slab gels. This method is highly labor-intensive and exposes users to hazardous chemicals such as ethidium bromide. Moreover, resolution of such gels is often poor and detailed analysis of the data in terms of fragment sizes and concentration can be tedious – especially when the data are to be compared with previously analyzed PCR products.
This part of the webinar series will focus on the challenges of typical genotyping applications and discuss how you can speed up your nucleic acid analysis by replacing slab-gel electrophoresis with automated capillary gel electrophoresis on the QIAxcel Advanced System while streamlining your entire multiplex PCR analysis.
Sign up for free to hear more from our experts and ask your questions during the Q&A session.