NGS is a fast-growing field, and advances in molecular barcoding, single cell sequencing, and more have made this technology more versatile than ever. Want to know what’s new? Join us for an exciting webinar series event this May – NGS Week @ QIAGEN! Our Global Product Manager for NGS, Dr. Samuel Rulli, will guide you through the latest advances in DNA-seq, RNA-seq, miRNA-seq, and more. Read the abstracts below to get more info!
NGS Week @ QIAGEN
May 22–26, 11 a.m. EDT; 8 a.m. PDT; 5 p.m. CEST
Introduction to Unique Molecular Index Targeted Sequencing (Monday, 5/22)
Errors related to the use of PCR in library construction have plagued targeted DNA and RNA sequencing experiments, calling into question the accuracy of results. Digital sequencing overcomes these challenges by introducing Unique Molecular Indices, or UMIs. These molecular barcodes are incorporated into the DNA or RNA sequences before any amplification takes place, eliminating errors such as PCR duplication and amplification bias. This webinar features an in-depth discussion of how UMI technology works and an introduction to how it is used in QIAGEN’s QIAseq sequencing solutions. Find out how to improve your NGS data with the accuracy of UMIs!
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Targeted DNA-seq for mutation detection (Tuesday, 5/23)
The utilization of targeted DNA sequencing with enrichment panels is on the rise for detecting genetic variants in cancers, inherited diseases and more. In this webinar, we introduce the QIAseq Targeted DNA Panels, which use Unique Molecular Indices (UMIs) and Single Primer Extension (SPE) technology to improve downstream analysis of difficult-to-sequence genes on Illumina and Thermo Fisher NGS instruments. QIAGEN uses its proprietary knowledge base to design panels for different cancer types, carrier testing, cardiomyopathy and other areas of interest. In addition, QIAGEN offers full coverage of the human genome, allowing researchers to build custom panels for their specific applications. Join us to find out how you can quickly and reliably detect genetic variants in your research from FFPE and fresh samples.
Gene expression analysis using targeted RNA-seq and desktop NGS instruments
Not every experiment requires whole transcriptome sequencing, but until recently, there hasn’t been a convenient way to profile transcriptomes in biological research areas such as cancer or immunity. In this webinar, we discuss the strategy and technology of targeted RNA sequencing, including the use of novel targeted RNA-seq panels covering the transcriptomes of 8 different pathways for human and mouse, as well as 170+ smaller panels. Using Single Primer Extension (SPE) and Unique Molecular Index (UMI) technology, the QIAseq panels overcome traditional RNA-seq limitations and ensure increased precision and accuracy. Join us and learn how you can easily upgrade your RNA-seq data and move beyond the limits of qPCR, microarray and traditional RNA-seq.
Linking miRNA and gene expression using NGS (Thursday, 5/25)
Total RNA discovery includes exploring both gene expression and regulation. This means analyzing both mRNA and noncoding regulatory RNA species such as microRNA. Until recently, successful miRNA-seq was difficult due to challenges such as contamination with adapter dimers and other RNA species, PCR bias and high sample input requirements. In this webinar, we discuss how new technological advances have helped overcome the challenges of miRNA-seq and RNA-seq, and why NGS is replacing qPCR as the technology of choice for total RNA discovery studies. Join us to find out about the recent advances in RNA sequencing and to learn about a case study in which NGS links microRNA with gene expression analysis.
Next-generation sequencing in single-cell applications (Friday, 5/26)
Single-cell sequencing is revolutionizing how we understand biology by revealing the individual contributions of cells rather than simply analyzing in bulk. QIAGEN has developed complete cell-to-library solutions for DNA and RNA sequencing libraries from single cells, as well as the novel QIAscout system for reliable single-cell isolation. In this webinar, we discuss several applications for single-cell NGS, along with application data. Find out how to apply NGS to your single-cell research!
We look forward to seeing you for NGS Week!