Working with DNA samples requires a lot of accuracy and precision. Anyone who has ever run a
polymerase chain reaction (PCR) is very aware of how time consuming and complex this
technique is. There are so many rules to follow and steps where potential mistakes can be
introduced. But, does it have to be that complicated? Is there any way to simplify your work? Is
the dream of confidently processing your PCR possible? We have an answer for you.
In our previous blog post, we introduced an extraordinarily easy solution for everyday PCR. In it,
we discussed AllTaq Master Mix and PCR Core Kits, providing ways to easily improve your lab
life and obtain better results. We mentioned our hot-start mechanism to ensure reagents remain
stable on the bench at room temperature, the new generation buffer system for unsurpassed
reliability and visual pipetting control with gel tracking dyes monitor successful procedure. While
all of that sounds great, you might wonder if this really works as well as presented. Is it just a
fairy tale? No need to wonder any more – we got fellow scientists to check it for you.
At the University of Nottingham, young scientists are working to find safe and effective therapy
for Clostridium difficile associated diseases. This microorganism is a highly dangerous
nosocomial pathogen and poses many serious problems. In the USA alone, there are around
250,000 patients infected by Clostridium difficile and approximately 14,000 deaths caused by
this pathogen every year (1). Despite repeated efforts, this remains a major challenge for
healthcare systems around the world. The solution might be bacteriophage (phage) therapy for
treatment of recurrent C. difficile infection based on their potential impact in the success of fecal
microbiota transplantation (2).
At the University of Nottingham, studies focused on better understanding the interaction between
phages and bacteria at the molecular level involve a great deal of PCR in their daily experiments.
In this video, you can hear about the main challenges they face in everyday work. Using the
proper conditions and adjusting procedures accordingly is one thing, but time is also taking a large
part of their research. AllTaq Kits help to improve and speed up their work. Offering the ability to
handle GC-rich sequences, visual pipetting control and removing ice from the equation allows
scientists to get results faster and more efficiently. When the data obtained from a study might
help thousands of people all over the world, every minute matters.
If you ever worked in a lab with DNA samples, you know how complex this process is and how
focused you need to be as even the smallest mistake might affect your results. Each solution
intent on making life in the lab easier is highly appreciated. We at QIAGEN understand this and
with this in mind, we developed AllTaq Kits – your unique solution for PCR to be covered with
1. Katherine R. Hargreaves, Martha R. J. Clokie (2014) Clostridium difficile phages: still
difficult? Front Microbiol.; 5: 184 (link)
2. Louis-Charles Fortier (2018) Bacteriophages Contribute to Shaping Clostridioides
(Clostridium) difficile Species. Front Microbiol.; 9: 2033 (link)