Biobanking

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Impact of storage buffer and temperature of stored DNA on PCR amplification products

In today’s clinical and translational research, blood is one of most common biospecimens being used. Although the highest DNA yield and quality is achieved by purifying genomic DNA from freshly harvested material, regretfully most of our precious samples cannot be processed straight away. That´s why sample management is so crucial right from the very start…. Read article →


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All you need is ONE – focusing on the unmet needs of single cell research

Browse through PubMed and perform a search with keywords like transcriptomics, proteomics, genomics, sequencing or amplification. Pick any paper answering questions in cancer research, neurobiology, stem cell biology, immunology or developmental biology. Are you amazed by how often you come across the term “single-cell”? Don’t be! The field of single-cell analysis has grown enormously in the recent years. Nature… Read article →


Quality control PCRD

The reproducibility crisis: what can we do?

There’s a growing trend of irreproducible results in biomedical research. In a previous post, Kurchi Bhattacharya reported on recent studies finding that a surprising number of scientists could not only not reproduce the results of their colleagues, but many found their own results difficult to reproduce as well. Much of the reproducibility problem likely has… Read article →


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Still using xylene to manually extract DNA from your FFPE samples?

For decades, formalin-fixed paraffin-embedded (FFPE) tissue samples have been collected and archived for long-term storage. They’re extremely valuable for carrying out research like mutation screening or pathogen detection. In general, deparaffinization of the FFPE tissue sections is achieved by manual xylene pretreatment, which dissolves the paraffin from the tissue, and a series of ethanol washes… Read article →


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How reliable are your samples?

Have you ever been in a situation where you prepared every necessary reagent for your experiment in the proper way, diligently performed every protocol step, and still ended up with background signals or even no signals in your gels, plots and blots? Have you conducted the experiment again while troubleshooting various components, but never found an answer? Wait – did you… Read article →