Exosomes & Microvesicles

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Phenol-based and non-phenol-based methods for extracting exosomal RNA

Studies in recent years have established that RNA contents in exosomes can serve as biomarkers for certain cancers (1). As exosomal RNA becomes more critical to research, successful isolation of these nanovesicles has become equally fundamental. Exosomes are small (40–100 nm) nanovesicles containing RNA that can move between cells. Found in several biological fluids, including blood plasma… Read article →



Exosomes

Is ultracentrifugation still the gold standard for exosome isolation?

There’s a new, proven method that may be knocking ultracentrifugation from its perch. Liquid biopsies and biomarker research depend on reliable access to exosomes and other extracellular vesicles (EVs), but standard ultracentrifugation-based protocols are laborious and don’t yield standardized results. Traditional ultracentrifugation-based protocols to isolate EVs are labor-intensive and subject to significant variability. Various attempts to… Read article →


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Maximizing quantity and purity of exosomal RNA for liquid biopsy

Exosomes are small membrane-bound vesicles ranging in size from 30 to 100 nm. They have been successfully isolated from multiple biofluids, including blood, plasma, urine and cerebrospinal fluid, and are being increasingly investigated for use in liquid biopsy. Although their primary function was once thought to be waste removal, further study has determined that exosomes… Read article →


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Big things in small packages for liquid biopsy: extracellular communication via exosomes

Cells utilize multiple mechanisms to send messages. Long-range signaling, the production of molecules from a single organ which then travel through the bloodstream to the rest of the body, is regulated by the endocrine system. Signaling molecules secreted by the endocrine system include steroid hormones, growth factors, glycoproteins and catecholamines. The list of endocrine organs… Read article →