microRNA Biomarkers

Biogenesis

miRNA-seq – when should you use it, and what are the challenges?

Exploring microRNA expression and function has led to some important discoveries about the regulation of gene expression in normal biological processes and disease states, and has even recently given rise to unique circulating biomarker signatures. qPCR and microarrays are the traditional techniques used to assess microRNA expression, and they have their strong points – qPCR… Read article →


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Missing half your microRNA sequencing data? This is how you get it back.

Want to discover a new circulating miRNA biomarker, or characterize the miRNome of a new species? microRNA sequencing is the future of gene regulation analysis, providing unmatched specificity for closely related microRNAs as well as the ability to detect novel noncoding transcripts. But challenges in the current workflow can cause up to half of microRNA… Read article →


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What is the future of microRNA technology?

Technology for profiling of microRNA by qPCR is ever evolving, opening up the opportunity for deeper insights into gene regulation. The development of locked nucleic acids (LNA), for example, enhanced qPCR primer specificity, while the design for an effective reverse transcription method for plant microRNAs opened up a new phylogenetic kingdom for qPCR profiling. Developing assays to… Read article →


Biogenesis

Back to the beginning: microRNA biogenesis

Introduction Since the discovery of microRNA (miRNA) in the early 1990s, much effort has been devoted to understanding how miRNAs are produced in the cell and how genes are controlled by these tiny RNAs. In today’s blog, we look back to the very beginning of miRNA discovery, and discuss miRNA biogenesis and its influence in cancer… Read article →


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The Bioscientist Webinar Festival – a pool of educational content

Here at QIAGEN, one of our main aims is to provide you with excellent, actionable resources that will support you in your research and experiments. As a result, we create content on a regular basis that can help you gain insights into important topics of scientific and specialized interest. As part of this ongoing effort,… Read article →