microRNA Biomarkers

QIAseq_miRNA_MCF_1000

New webinar: QIAseq technology overcomes miRNA-seq challenges

As a microRNA detection technology, microRNA sequencing (miRNA-seq) has the potential to uncover new microRNAs, identify processing intermediates and reveal quantitative differences between samples. However, challenges presented by FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis have led to disappointing and often irreproducible results. This Wednesday,… Read article →



Biogenesis

miRNA-seq – when should you use it, and what are the challenges?

Exploring microRNA expression and function has led to some important discoveries about the regulation of gene expression in normal biological processes and disease states, and has even recently given rise to unique circulating biomarker signatures. qPCR and microarrays are the traditional techniques used to assess microRNA expression, and they have their strong points – qPCR… Read article →


QIAseq_miRNA_MCF_1000

Missing half your microRNA sequencing data? This is how you get it back.

Want to discover a new circulating miRNA biomarker, or characterize the miRNome of a new species? microRNA sequencing is the future of gene regulation analysis, providing unmatched specificity for closely related microRNAs as well as the ability to detect novel noncoding transcripts. But challenges in the current workflow can cause up to half of microRNA… Read article →


LungCancer+miRNA_700x233

What is the future of microRNA technology?

Technology for profiling of microRNA by qPCR is ever evolving, opening up the opportunity for deeper insights into gene regulation. The development of locked nucleic acids (LNA), for example, enhanced qPCR primer specificity, while the design for an effective reverse transcription method for plant microRNAs opened up a new phylogenetic kingdom for qPCR profiling. Developing assays to… Read article →