NGS Pre-Analytics


Biogenesis

Eliminating contaminants in miRNA-seq libaries just got easier

microRNA sequencing is not only useful for finding novel microRNAs and detecting isomiRs; it’s also an attractive method for miRNA expression profiling, particularly when combined with molecular indexing to boost quantification accuracy (see our previous post, Barcodes in NGS: sample vs molecular). But preparing a library to sequence purely microRNAs can be difficult due to contaminating… Read article →


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Join our webinar: State-of-the-art NGS library quality control solutions

Next-generation sequencing (NGS) is revolutionizing life science research and healthcare. The advent of multiple platforms has opened the doors to increased throughput, speed, sensitivity and flexibility, providing researchers with valuable insights at an unprecedented rate. While recent technological developments have made NGS more accessible to smaller labs, many barriers to implementation still remain. NGS workflows… Read article →


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Messengers at the junction of inflammation and cancer: exosomes

The more we learn about exosomes, the more we realize just how powerful they are in cancer. As agents of cell-to-cell communication, exosomes play important roles in tumorigenesis, cancer progression, and metastasis. One of the most interesting areas in which exosomes are exerting their influence is at the interface of cancer and another key process… Read article →


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New webinar: QIAseq technology overcomes miRNA-seq challenges

As a microRNA detection technology, microRNA sequencing (miRNA-seq) has the potential to uncover new microRNAs, identify processing intermediates and reveal quantitative differences between samples. However, challenges presented by FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis have led to disappointing and often irreproducible results. This Wednesday,… Read article →