PCR Solutions

ILLU_0694_Multiplex (1)

Quantitative readout of multiplex PCR assays using synthetic templates

Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →


ILLU_0694_Multiplex (1)

Getting Multiplex PCR gene expression profiling working for you

Multiplex PCR is an extremely enabling molecular biology technique, allowing the amplification of multiple targets in a single PCR experiment. There are a broad range of research applications for multiplex PCR, including pathogen identification, high-throughput SNP genotyping, gene expression profiling, template quantitation, RNA detection and even forensic studies. Gene expression profiling of CTCs  The presence… Read article →



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Effective genomic DNA removal – crucial for accurate gene expression analysis

Whether you are working with formalin-fixed, paraffin embedded (FFPE) tissue or normal tissue samples, the elimination of genomic DNA (gDNA) from your RNA preps is crucial to obtain reliable results in gene expression analysis, especially if you want to determine the gene expression pattern in a specific cell or under specific conditions. Possible consequences of… Read article →


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Macrauchenia gets its place on the tree of life – thanks to ancient DNA analysis

Original photo courtesy of Ryan Somma. This file is licensed under the Creative Commons. Macrauchenia was a camel-like animal deriving its name from Auchenia, meaning “big neck” in Greek, and its first remains, which date back 7 million years, were found in South America, primarily in Argentina (1). Macrauchenia was a herbivore with its distinctive feature being… Read article →