Bisulfite conversion is the most popular method for DNA methylation analysis and the most convenient and effective way to map DNA methylation to individual bases. Yet there are many things to consider in order for you to get reliable results. In particular, the bisulfite treatment of DNA and the resulting conversion of unmethylated cytosines to… Read article →
Of all the methods used for gene expression studies, RT-qPCR is still the gold standard for accurate quantification of transcripts. The accuracy of RT-qPCR, as well as its accessibility, also makes it the logical next step in biomarker discovery studies following a broader RNA-seq survey of the messenger and/or noncoding RNAs in a system. What… Read article →
Market research shows that 66.6% of researchers use qRT-PCR for gene expression profiling. The popularity of this technique speaks to its effectiveness for detecting messenger RNA levels, but qRT-PCR can still be prone to pitfalls that sometimes lead to disappointing results. To help you improve your experiments, we’ve made a new infographic with interesting facts… Read article →
The immune system is an intricate host defense system of unique, yet highly coordinated responses to pathogens. The immune response is divided into two phases – innate and adaptive. Innate immunity provides the first line of defense through different families of pattern-recognition receptors (PRRs), such as Toll-like receptors (TLRs). Adaptive immunity, in the form of… Read article →
As a novel class of RNA, long noncoding RNA (lncRNA) plays important roles in gene regulation via a variety of mechanisms. Current research is deepening our understanding of the complex and sometimes contradictory roles of lncRNA. For example, lncRNAs can compete with transcription, but at other times they can support transcription, recruiting specific epigenetic factors… Read article →
04/23/2018
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