Sometimes our lives get so busy that we don’t always appreciate the little things, such as enjoying a hot cup of tea on a sunny afternoon, or receiving a kind “thank you´´ from a colleague. It’s the same in research: there are multiple examples where the little things that matter the most get overlooked. miRNA… Read article →
Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →
Multiplex PCR is an extremely enabling molecular biology technique, allowing the amplification of multiple targets in a single PCR experiment. There are a broad range of research applications for multiplex PCR, including pathogen identification, high-throughput SNP genotyping, gene expression profiling, template quantitation, RNA detection and even forensic studies. Gene expression profiling of CTCs The presence… Read article →
A dementia diagnosis can be devastating – not only for the person with the disease, but also for their loved ones. For people who have never experienced dementia, forgetfulness might be interpreted as an endearing sign of seniority, but after a certain stage at which a person may not remember familiar people, their surroundings, or even… Read article →
Whether you are working with formalin-fixed, paraffin embedded (FFPE) tissue or normal tissue samples, the elimination of genomic DNA (gDNA) from your RNA preps is crucial to obtain reliable results in gene expression analysis, especially if you want to determine the gene expression pattern in a specific cell or under specific conditions. Possible consequences of… Read article →
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