Multiplex PCR is an extremely enabling molecular biology technique, allowing the amplification of multiple targets in a single PCR experiment. There are a broad range of research applications for multiplex PCR, including pathogen identification, high-throughput SNP genotyping, gene expression profiling, template quantitation, RNA detection and even forensic studies. Gene expression profiling of CTCs The presence… Read article →
A dementia diagnosis can be devastating – not only for the person with the disease, but also for their loved ones. For people who have never experienced dementia, forgetfulness might be interpreted as an endearing sign of seniority, but after a certain stage at which a person may not remember familiar people, their surroundings, or even… Read article →
Whether you are working with formalin-fixed, paraffin embedded (FFPE) tissue or normal tissue samples, the elimination of genomic DNA (gDNA) from your RNA preps is crucial to obtain reliable results in gene expression analysis, especially if you want to determine the gene expression pattern in a specific cell or under specific conditions. Possible consequences of… Read article →
Original photo courtesy of Ryan Somma. This file is licensed under the Creative Commons. Macrauchenia was a camel-like animal deriving its name from Auchenia, meaning “big neck” in Greek, and its first remains, which date back 7 million years, were found in South America, primarily in Argentina (1). Macrauchenia was a herbivore with its distinctive feature being… Read article →
When working hard to complete urgent experiments, it’s easy to overlook the basics, whether you’re an expert or complete beginner. One basic but critical factor to consider for sensitive downstream applications, such as qPCR, is the quality of extracted RNA. Successful extraction of high-quality RNA, especially from some difficult sample types, can be a challenge…. Read article →
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