PCR Solutions

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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →

Quality control PCRD

The reproducibility crisis: what can we do?

There’s a growing trend of irreproducible results in biomedical research. In a previous post, Kurchi Bhattacharya reported on recent studies finding that a surprising number of scientists could not only not reproduce the results of their colleagues, but many found their own results difficult to reproduce as well. Much of the reproducibility problem likely has… Read article →

Plasmid eGuide

Get the most from your plasmids – the eGuide way!

In 1986, the launch of the QIAGEN Plasmid Maxi Purification Kit meant an incredible acceleration in time to result: from 2 days to just 2 hours! Now our 73 QIAGEN Plasmid family members are used worldwide for remarkable research. To help meet the need for easy selection of the correct tool to optimize your plasmid purification… Read article →


The PD1 race picks up pace

If you’ve been following developments in cancer immunotherapy closely, you’ve probably heard of programmed death 1 (PD1/PDCD1, also known as CD279) and PDL1 (CD274). Their interaction is at the center of new immune checkpoint-targeting therapies coming out of the pharmaceutical industry, such as Bristol-Myers Squibb’s nivolumab and Merck’s pembrolizumab (1, 5). What are PD1 and PDL1, and… Read article →


qPCR from start to finish – get our new technical guide

Over the years, we’ve offered a lot of presentations, and one of the most consistently well-attended is the Introduction to qPCR (view the recording). That’s no surprise – qPCR remains one of the most widely-used techniques for gene expression analysis, offering wide dynamic range, great sensitivity and specificity, and accessibility for most researchers. Now, as a… Read article →