Have you checked out the “What’s New” section of Current Protocols in Molecular Biology? Two new protocols are up for analysis of small cell numbers – I think you’ll find them useful! The first one is a step-by-step single cell protocol that takes you through the whole workflow from a single cell to NGS library preparation,… Read article →
Single Cell Analysis
Do you wonder what you might find if you narrowed your genomic and transcriptomic analyses down to the single-cell level? If you suppressed the noise from ten thousand other cells, what might an individual cell have to say? Single-cell analysis is a burgeoning area of interest, spurred by advances in sequencing technology. Down to the… Read article →
Whether you’re studying single cell genomics, circulating tumor cells or the microbiome, analyzing single cells creates the potential for a deeper understanding of cell heterogeneity. But several challenges stand in the way of this goal, such as allelic dropout, coverage uniformity and low yield. How can you overcome these? The single cell team at QIAGEN… Read article →
Are you working with single cells? Find out what your fellow scientists are doing in this area by completing the global single cell analysis survey. If you’d like to receive a summary of the results, just complete the contact information section and you’ll get the summary in about 3 weeks. Take the survey!
Deciding on which type of analysis to use is key for any single cell experiment. When should you use whole-genome and whole-transcriptome profiling, and when is it right to use targeted profiling instead? Whole genome sequencing (WGS) is the method of choice if you have a lot of resources and time. WGS enables the study… Read article →