Single Cell Analysis

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Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →



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Overcoming coverage bias in single-cell whole genome sequencing (WGS)

Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →


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Detection of chromosomal rearrangements

Recently in Nature, Cheng-Zhong Zhang and colleagues published a very interesting approach that provides the first direct experimental evidence for a mechanism leading to chromothripsis (1). What exactly is chromothripsis? Zhang’s publication piqued my interest to dig a bit deeper into this phenomenon. Chromothripsis is a kind of catastrophic mutational event that results in massive rearrangements… Read article →