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Single Cell Analysis
Deciding on which type of analysis to use is key for any single cell experiment. When should you use whole-genome and whole-transcriptome profiling, and when is it right to use targeted profiling instead? Whole genome sequencing (WGS) is the method of choice if you have a lot of resources and time. WGS enables the study… Read article →
In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →
Are you working with single cells or a very low number of cells and want to analyze by next-generation sequencing? Well, we do too. However, challenges like small sample amounts and the heterogeneity of the input materials make designing the right method critical to effective analysis. What’s the best way to conduct genomic and transcriptomic… Read article →
Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →