Single Cell Analysis

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Global survey on single cell analysis launched

Are you working with single cells? Find out what your fellow scientists are doing in this area by completing the global single cell analysis survey. If you’d like to receive a summary of the results, just complete the contact information section and you’ll get the summary in about 3 weeks. Take the survey!



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Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →



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Overcoming coverage bias in single-cell whole genome sequencing (WGS)

Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →