Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →
Cell-free circulating tumor DNA (ctDNA) is fragmented DNA from tumors (160–180 base pairs) that is found in the cell-free part of whole blood (i.e., plasma and serum) (1, 2). ctDNA can be distinguished from normal cell-free DNA (cfDNA) by the presence of somatic mutations (2). Analysis of ctDNA has the potential to be a ‘liquid… Read article →
Bacteremia is the presence of bacteria in the blood. Under normal circumstances, blood should be sterile, but with the insertion of a catheter into the body or through an open wound, bacteria can gain entry and take hold. Even small numbers of bacteria can be a real problem and make people very sick. Untreated bacteremia… Read article →
When I recently came across this new article in Nature (1), it got me thinking back to the days when I worked in the lab, studying Clostridium perfringens and chickens using carvacrol. Essential oils (EOs) have been known for their antibacterial properties for some time now. Major components of EOs, like thymol and carvacrol, are… Read article →
Multiplex PCR is an extremely enabling molecular biology technique, allowing the amplification of multiple targets in a single PCR experiment. There are a broad range of research applications for multiplex PCR, including pathogen identification, high-throughput SNP genotyping, gene expression profiling, template quantitation, RNA detection and even forensic studies. Gene expression profiling of CTCs The presence… Read article →
09/26/2017
Check out this series on automated solutions for microbiome research, methylation studies and more, featuring the QIAcube, Rotor-Gene Q and the new PyroMark Q48 Autoprep.
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Learn about our new sample prep solutions for your microbiome research at booth 921, Hall 4 at #IBMSCongress2017:… https://t.co/fb8UEDWmhD, Detect & quantify microbes in your samples easily! Stop by booth 921, Hall 4 to learn how #IBMSCongress2017:… https://t.co/l5OhjjD2IV, Arrived in Birmingham for #IBMSCongress2017? Visit #QIAGEN at booth 921, Hall 4 for Sample to Insight solutions:… https://t.co/cKw3nqEIyG, Upcoming webinar: Single-cell RNA-seq – linking cell morphology to transcriptomics. Register for Sept 25:… https://t.co/A38YaMQZzB, 
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