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New videos on single cell analysis

Whether you’re studying single cell genomics, circulating tumor cells or the microbiome, analyzing single cells creates the potential for a deeper understanding of cell heterogeneity. But several challenges stand in the way of this goal, such as allelic dropout, coverage uniformity and low yield. How can you overcome these? The single cell team at QIAGEN… Read article →


Epigenetics

Location, location, location: methylation analysis for epigenetic research

No, we’re not talking about real estate! In research, location is key to how DNA methylation works. Epigenetics, the study of chromatin-modifying marks that do not alter DNA sequences, has recently undergone a paradigm shift regarding the effect of DNA methylation on gene transcription. In the human genome, the most commonly studied epigenetic mark is 5-methylcytosine, often… Read article →


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How pure is pure enough for DNA in NGS?

DNA purification is a critical step for obtaining accurate NGS data, because the quality of your starting material determines the reliability of next-generation sequencing results and data analysis. Even the best and most robust NGS technologies can only provide reliable results if the template nucleic acid is of adequate quality and purity. DNA purity is especially important at target… Read article →


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A webinar with Dr. Jo Vandesompele – Expanding the dynamic range of microRNA expression

In 2014, an ambitious microRNA technology study was published in Nature Methods. This study evaluated the existing microRNA platforms on the market for their technical performance. The authors, including first author Pieter Mestdagh and corresponding author Jo Vandesompele of Ghent University and Biogazelle, found small differences between the qPCR technologies, but concluded that from this purely technical perspective, there was no… Read article →