Cryopreservation (cryogenic preservation) is “the storage of cells, tissues and organs following appropriate ‘preparatory’ procedures at the ultra-low temperature of liquid nitrogen (–196°C).” (1) This form of preservation is ideal for biobanking because these cold temperatures slow chemical and physical reactions of the biomolecules and biospecimens and stop the samples from degrading. Liquid nitrogen biobanks… Read article →
Are you working with single cells? Find out what your fellow scientists are doing in this area by completing the global single cell analysis survey. If you’d like to receive a summary of the results, just complete the contact information section and you’ll get the summary in about 3 weeks. Take the survey!
Deciding on which type of analysis to use is key for any single cell experiment. When should you use whole-genome and whole-transcriptome profiling, and when is it right to use targeted profiling instead? Whole genome sequencing (WGS) is the method of choice if you have a lot of resources and time. WGS enables the study… Read article →
High-quality biobanked samples closely resemble the biospecimen prior to extraction, with minimal modification due to collection or storage conditions. Establishment of standard operating procedures (SOPs) is crucial to avoid introducing variables into your research samples during storage and maintain the high quality required for reliable results. According to current recommendations, there are 12 SOPs that… Read article →
It’s hard to overstate the importance of amplification technology in molecular biology. Reverse transcription-polymerase chain reaction (RT-PCR) amplifies sections of messenger RNA so they can be quantified – we’d have a much dimmer view of the transcriptome without it. Whole-genome amplification helps us analyze genes in single or limited cells, opening up a whole new… Read article →