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CTC enrichment: methods and critical steps

Circulating tumor cells (CTCs), cells that have detached from the primary tumor and entered the peripheral blood, are emerging as a significant source of genetic material for clinical guidance. However, the relative rarity of these cells, between 1–10 cells per 10 ml in most patients (1), makes it challenging to unlock their potential. Separating CTCs… Read article →


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The original human microRNA – the let-7 family as cancer biomarkers

The human genome isn’t short on microRNAs. More than 2500 have been identified, and they affect a huge number of biological pathways, including immunity, cell death, development and more. Moreover, as we’ve discussed a number of times on this blog, microRNAs are increasingly scrutinized in translational research for their potential as diagnostic, prognostic and therapeutic… Read article →


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Single-cell sequencing – how should you construct your libraries?

For the past several decades, due to technical limitations, the field of genomics and transcriptomics has focused on population-level measurements that sometimes ignore the significant differences between individual cells. With advances in whole genome amplification (WGA), whole transcriptome amplification (WTA) and NGS technologies, it is now possible to profile the responses of individual cells at… Read article →



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Molecular characterization of circulating tumor cells

Although circulating tumor cells (CTCs) hold a wealth of information, the only clinically approved use of CTCs is enumeration for the prediction of progression-free survival and overall survival. The use of CTCs for prognosis is groundbreaking, but specifically enriching and counting tumor cells does not fully exploit the information available in CTCs. A real‑world analogy… Read article →