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Maximizing quantity and purity of exosomal RNA for liquid biopsy

Exosomes are small membrane-bound vesicles ranging in size from 30 to 100 nm. They have been successfully isolated from multiple biofluids, including blood, plasma, urine and cerebrospinal fluid, and are being increasingly investigated for use in liquid biopsy. Although their primary function was once thought to be waste removal, further study has determined that exosomes… Read article →


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Big things in small packages for liquid biopsy: extracellular communication via exosomes

Cells utilize multiple mechanisms to send messages. Long-range signaling, the production of molecules from a single organ which then travel through the bloodstream to the rest of the body, is regulated by the endocrine system. Signaling molecules secreted by the endocrine system include steroid hormones, growth factors, glycoproteins and catecholamines. The list of endocrine organs… Read article →


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Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →