Big things in small packages for liquid biopsy: extracellular communication via exosomes

Cells utilize multiple mechanisms to send messages. Long-range signaling, the production of molecules from a single organ which then travel through the bloodstream to the rest of the body, is regulated by the endocrine system. Signaling molecules secreted by the endocrine system include steroid hormones, growth factors, glycoproteins and catecholamines. The list of endocrine organs… Read article →


Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →

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Brainy exosomes: a novel pool for biomarker discovery in human CSF

Exosomes, endocytic vesicles ranging in size from 40–100nm, have revolutionized the field of cell–cell communication. Although they were first described in the 1980s, detailed analysis of exosome biogenesis, composition and function has occurred primarily within the past decade. The significance of exosomes is enhanced by the fact that exosomes transport packets of cellular information rather… Read article →