DNA purification is a critical step for obtaining accurate NGS data, because the quality of your starting material determines the reliability of next-generation sequencing results and data analysis. Even the best and most robust NGS technologies can only provide reliable results if the template nucleic acid is of adequate quality and purity. DNA purity is especially important at target… Read article →
In 2014, an ambitious microRNA technology study was published in Nature Methods. This study evaluated the existing microRNA platforms on the market for their technical performance. The authors, including first author Pieter Mestdagh and corresponding author Jo Vandesompele of Ghent University and Biogazelle, found small differences between the qPCR technologies, but concluded that from this purely technical perspective, there was no… Read article →
Cryopreservation (cryogenic preservation) is “the storage of cells, tissues and organs following appropriate ‘preparatory’ procedures at the ultra-low temperature of liquid nitrogen (–196°C).” (1) This form of preservation is ideal for biobanking because these cold temperatures slow chemical and physical reactions of the biomolecules and biospecimens and stop the samples from degrading. Liquid nitrogen biobanks… Read article →
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Deciding on which type of analysis to use is key for any single cell experiment. When should you use whole-genome and whole-transcriptome profiling, and when is it right to use targeted profiling instead? Whole genome sequencing (WGS) is the method of choice if you have a lot of resources and time. WGS enables the study… Read article →