MS_0039_PAXgeneTissueMIX_B2

Technology for preservation of nucleic acids and morphology in tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common specimen available for molecular assays on tissue after diagnostic histopathological examination (1). These tissue samples can also provide valuable sources of stable nucleic acids for research as part of biobanks, but poor recovery and quality of nucleic acids from FFPE tissue specimens often affects the accuracy and… Read article →


Single_Cell_700x233

Overcoming coverage bias in single-cell whole genome sequencing (WGS)

Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →


Stress+Toxicity_700x233

Environmental toxin biomarkers

What impact do environmental contaminants have on human health? Until recently, it was difficult to adequately address this complex question. From animal models and cell and tissue culture studies, it has been clear for quite some time that contaminants ranging from heavy metals to organic chemicals like BPA, PCBs and dioxins can be extremely detrimental… Read article →


LungCancer+miRNA_700x233

Cancer in the lungs? The blood will tell – miRNA in non-small cell lung cancer

In a previous post, we discussed how small non-coding RNAs could be identified to serve as circulating biomarkers in lung cancer. Now, a new study from Ma et al. defines a promising microRNA signature in peripheral blood mononuclear cells. The signature can distinguish non-small cell lung carcinoma (NSCLC) with 72.62% sensitivity and 82.61% specificity, and squamous… Read article →


Exosomes_700x233

Tumor-derived exosomes

Tumor cells utilize multiple signaling mechanisms to modify surrounding tissue and create a favorable environment for growth and metastasis. Direct cell–cell communication is mediated by gap junctions, which are specialized channels that allow the transfer of ions and small molecules between adjacent cells. Paracrine signaling molecules, such as TGF-beta and fibroblast growth factor, act over a… Read article →