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Critical factors for successful molecular analysis of FFPE samples

Formalin fixation and paraffin embedding (FFPE) can impair the quality of nucleic acids obtained from tissue samples. The molecular analysis of FFPE samples is negatively affected by the low quality of analytes from FFPE samples. Taking this into consideration is important when performing successful molecular analyses. In this post, we’ll discuss how FFPE affects RNA… Read article →


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Technology for preservation of nucleic acids and morphology in tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common specimen available for molecular assays on tissue after diagnostic histopathological examination (1). These tissue samples can also provide valuable sources of stable nucleic acids for research as part of biobanks, but poor recovery and quality of nucleic acids from FFPE tissue specimens often affects the accuracy and… Read article →


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Overcoming coverage bias in single-cell whole genome sequencing (WGS)

Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →


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Environmental toxin biomarkers

What impact do environmental contaminants have on human health? Until recently, it was difficult to adequately address this complex question. From animal models and cell and tissue culture studies, it has been clear for quite some time that contaminants ranging from heavy metals to organic chemicals like BPA, PCBs and dioxins can be extremely detrimental… Read article →


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Cancer in the lungs? The blood will tell – miRNA in non-small cell lung cancer

In a previous post, we discussed how small non-coding RNAs could be identified to serve as circulating biomarkers in lung cancer. Now, a new study from Ma et al. defines a promising microRNA signature in peripheral blood mononuclear cells. The signature can distinguish non-small cell lung carcinoma (NSCLC) with 72.62% sensitivity and 82.61% specificity, and squamous… Read article →