Posts Tagged: DNA

Epigenetics

Epigenetics research and its significance in cancer

Introduction to epigenetics and techniques There has been a great interest in the study of epigenetic mechanisms and DNA methylation in many areas of research, including DNA repair, cell cycle control, developmental biology, cancer research, identification of biomarkers, predisposition factors and potential drug targets. Lately I’ve been immersed in this field via my close work… Read article →


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Novel digital sequencing technology promises to advance oncology research

As research uncovers the underlying mutations and mechanisms that drive oncogenesis and cancer progression, it’s becoming clearer that cures are likely to come not from a single silver bullet, but from identifying each individual’s personal cancer drivers. Next-generation sequencing (NGS) is the leading strategy for finding sequence variants and fusion genes responsible for cancer, and targeted… Read article →


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Expert talks how to purify more DNA from challenging tissues

Mechanical and enzymatic lysis both have their drawbacks when isolating genomic DNA from solid tissue samples. While mechanical disruption tends to provide high yields from tissue samples, it does damage the nucleic acids. Enzymatic lysis, like Proteinase K digestion, allows extraction of nucleic acids without damage, but requires long incubation times and provides lower yields… Read article →


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Quantitative readout of multiplex PCR assays using synthetic templates

Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →


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Still using xylene to manually extract DNA from your FFPE samples?

For decades, formalin-fixed paraffin-embedded (FFPE) tissue samples have been collected and archived for long-term storage. They’re extremely valuable for carrying out research like mutation screening or pathogen detection. In general, deparaffinization of the FFPE tissue sections is achieved by manual xylene pretreatment, which dissolves the paraffin from the tissue, and a series of ethanol washes… Read article →