Posts Tagged: DNA

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Quantitative readout of multiplex PCR assays using synthetic templates

Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →



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Webinars on-demand: Digital NGS for RNA-seq, miRNA-seq and DNA-seq!

Digital NGS using Unique Molecular Indices (UMIs) is a game-changer in RNA-seq, miRNA-seq and DNA-seq, enabling accurate quantification by overcoming PCR bias. In May, we presented the details of this technology for gene expression, microRNA analysis and mutation detection as part of our NGS Week webinar series, and again in July as part of the Summer of… Read article →



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Effective genomic DNA removal – crucial for accurate gene expression analysis

Whether you are working with formalin-fixed, paraffin embedded (FFPE) tissue or normal tissue samples, the elimination of genomic DNA (gDNA) from your RNA preps is crucial to obtain reliable results in gene expression analysis, especially if you want to determine the gene expression pattern in a specific cell or under specific conditions. Possible consequences of… Read article →