Posts Tagged: DNA


NGS live webinar: an innovative large cohort study for breast cancer risk – sequencing 60,000 samples

Next-generation sequencing (NGS) is being utilized for numerous new and exciting applications. One application includes analyzing the genetic variants of targeted genes that are associated with cancer development to assess cancer risk. Targeted DNA sequencing with a focused set of genes is a powerful and widely used approach to detect low-frequency variants. However, researchers using… Read article →


What are the keys to successful bisulfite conversion?

Bisulfite conversion is the most popular method for DNA methylation analysis and the most convenient and effective way to map DNA methylation to individual bases. Yet there are many things to consider in order for you to get reliable results. In particular, the bisulfite treatment of DNA and the resulting conversion of unmethylated cytosines to… Read article →

gDNA isolation

What are the top factors in successful gDNA isolation and quality control?

The quality of extracted genomic DNA (gDNA) is a critical factor in numerous downstream applications, such as next-generation sequencing (NGS) and real-time PCR. gDNA isolation from certain sample types can be challenging, though, and getting back to the basics of purification and quality control can help us understand how to best approach these challenges. QIAGEN’s… Read article →