Posts Tagged: FFPE


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Still using xylene to manually extract DNA from your FFPE samples?

For decades, formalin-fixed paraffin-embedded (FFPE) tissue samples have been collected and archived for long-term storage. They’re extremely valuable for carrying out research like mutation screening or pathogen detection. In general, deparaffinization of the FFPE tissue sections is achieved by manual xylene pretreatment, which dissolves the paraffin from the tissue, and a series of ethanol washes… Read article →


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One spin, one dedicated kit – selection wheels make it possible

It can be a real struggle sometimes to find and select the right isolation kit, whether it is for RNA or genomic DNA (gDNA) purification. Not using the right kit for your experiments has major consequences, however – the impact could be as severe as not being able to publish your results. Or worse, finding that other researchers were able… Read article →



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Understanding what factors contribute to variations in quantity of DNA purified from FFPE materials

QIAGEN recently presented a webinar exploring what factors contribute to variations in quantity of DNA purified from FFPE material. As noted in “Top 5 considerations for preparing and archiving FFPE samples,” FFPE is a standard method for long-term preservation of DNA in tissue biopsies. It’s critical to understand how to overcome the challenges that this sample type presents in order… Read article →