Posts Tagged: FFPE

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Understanding what factors contribute to variations in quantity of DNA purified from FFPE materials

QIAGEN recently presented a webinar exploring what factors contribute to variations in quantity of DNA purified from FFPE material. As noted in “Top 5 considerations for preparing and archiving FFPE samples,” FFPE is a standard method for long-term preservation of DNA in tissue biopsies. It’s critical to understand how to overcome the challenges that this sample type presents in order… Read article →


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Why preamplify in miRNA research? Download the white paper

It’s hard to overstate the importance of amplification technology in molecular biology. Reverse transcription-polymerase chain reaction (RT-PCR) amplifies sections of messenger RNA so they can be quantified – we’d have a much dimmer view of the transcriptome without it. Whole-genome amplification helps us analyze genes in single or limited cells, opening up a whole new… Read article →


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Critical factors for successful molecular analysis of FFPE samples

Formalin fixation and paraffin embedding (FFPE) can impair the quality of nucleic acids obtained from tissue samples. The molecular analysis of FFPE samples is negatively affected by the low quality of analytes from FFPE samples. Taking this into consideration is important when performing successful molecular analyses. In this post, we’ll discuss how FFPE affects RNA… Read article →


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New webinar shows synergy between two miRNA detection techniques

In our last post, we discussed the strengths and weaknesses of the three most popular techniques for miRNA detection, qPCR, miRNA-seq and microarray. We ultimately concluded that these miRNA detection technologies are appropriate for different experimental circumstances, and a lab’s decision about which to use should be driven by their research goals. Today, I’m excited to report on a presentation about… Read article →


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Critical factors in the retrieval of usable analytes from FFPE samples

The process of retrieving analytes from formalin-fixed paraffin-embedded (FFPE) tissue samples is an incredibly complicated endeavor. There are several major challenges when purifying DNA, RNA and protein from FFPE samples. First, these biomolecules are crosslinked to each other. Second, nucleic acids are often fragmented depending how the FFPE samples are prepared, and third, there is… Read article →