Posts Tagged: microRNA

Biogenesis

Eliminating contaminants in miRNA-seq libaries just got easier

microRNA sequencing is not only useful for finding novel microRNAs and detecting isomiRs; it’s also an attractive method for miRNA expression profiling, particularly when combined with molecular indexing to boost quantification accuracy (see our previous post, Barcodes in NGS: sample vs molecular). But preparing a library to sequence purely microRNAs can be difficult due to contaminating… Read article →


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RNAs that make your heart beat

Love, life, health and vitality. All are connected through one symbol: the beating of a heart. Often we don’t notice the strength of this organ until it starts to beat fast enough in our chests that we can feel it. One of the most beautiful instances of a racing heart happens during social bonding and when we fall in… Read article →


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New webinar: QIAseq technology overcomes miRNA-seq challenges

As a microRNA detection technology, microRNA sequencing (miRNA-seq) has the potential to uncover new microRNAs, identify processing intermediates and reveal quantitative differences between samples. However, challenges presented by FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis have led to disappointing and often irreproducible results. This Wednesday,… Read article →



Biogenesis

miRNA-seq – when should you use it, and what are the challenges?

Exploring microRNA expression and function has led to some important discoveries about the regulation of gene expression in normal biological processes and disease states, and has even recently given rise to unique circulating biomarker signatures. qPCR and microarrays are the traditional techniques used to assess microRNA expression, and they have their strong points – qPCR… Read article →