Posts Tagged: miRNA

Biogenesis

miRNA-seq vs qPCR in 2017: an update

Time marches on and technology with it, and these new advances yield new perspectives. Of particular interest to this blog is the changing technology and perspective on microRNA profiling methods. Two years ago, I wrote about the pros and cons of 3 methods commonly used for miRNA expression analysis, NGS, qPCR and microarray, and concluded… Read article →


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QIAGEN at the AACR Annual Meeting 2017 – innovations in NGS and more!

Graced by beautiful spring weather in Washington, D.C., the AACR Annual Meeting 2017 brought together scientists from around the globe to share insights into the latest and most exciting cancer research. At the QIAGEN booth, our colleagues shared the latest in QIAGEN innovations, including the revolutionary QIAscout single-cell isolation system, and spoke to customers about NGS,… Read article →


miRNAs+Barcodes_V01

Webinar highlights – Maximizing miRNA-seq success from liquid biopsy samples

Development of markers that facilitate earlier and non-invasive diagnosis is a primary goal of disease research. This is where microRNAs come in. Studies have indicated that the expression levels of these non-coding RNA molecules are altered in disease states, including cancer and neurodegeneration. Evidence of their presence in biofluids means that their potential as important… Read article →



Biogenesis

Eliminating contaminants in miRNA-seq libaries just got easier

microRNA sequencing is not only useful for finding novel microRNAs and detecting isomiRs; it’s also an attractive method for miRNA expression profiling, particularly when combined with molecular indexing to boost quantification accuracy (see our previous post, Barcodes in NGS: sample vs molecular). But preparing a library to sequence purely microRNAs can be difficult due to contaminating… Read article →