Posts Tagged: miRNA

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New webinar: QIAseq technology overcomes miRNA-seq challenges

As a microRNA detection technology, microRNA sequencing (miRNA-seq) has the potential to uncover new microRNAs, identify processing intermediates and reveal quantitative differences between samples. However, challenges presented by FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis have led to disappointing and often irreproducible results. This Wednesday,… Read article →



Biogenesis

miRNA-seq – when should you use it, and what are the challenges?

Exploring microRNA expression and function has led to some important discoveries about the regulation of gene expression in normal biological processes and disease states, and has even recently given rise to unique circulating biomarker signatures. qPCR and microarrays are the traditional techniques used to assess microRNA expression, and they have their strong points – qPCR… Read article →


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What is the future of microRNA technology?

Technology for profiling of microRNA by qPCR is ever evolving, opening up the opportunity for deeper insights into gene regulation. The development of locked nucleic acids (LNA), for example, enhanced qPCR primer specificity, while the design for an effective reverse transcription method for plant microRNAs opened up a new phylogenetic kingdom for qPCR profiling. Developing assays to… Read article →


Selection kit header

One spin, one dedicated kit – selection wheels make it possible

It can be a real struggle sometimes to find and select the right isolation kit, whether it is for RNA or genomic DNA (gDNA) purification. Not using the right kit for your experiments has major consequences, however – the impact could be as severe as not being able to publish your results. Or worse, finding that other researchers were able… Read article →