Posts Tagged: NGS


Biogenesis

Eliminating contaminants in miRNA-seq libaries just got easier

microRNA sequencing is not only useful for finding novel microRNAs and detecting isomiRs; it’s also an attractive method for miRNA expression profiling, particularly when combined with molecular indexing to boost quantification accuracy (see our previous post, Barcodes in NGS: sample vs molecular). But preparing a library to sequence purely microRNAs can be difficult due to contaminating… Read article →


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Join our webinar: State-of-the-art NGS library quality control solutions

Next-generation sequencing (NGS) is revolutionizing life science research and healthcare. The advent of multiple platforms has opened the doors to increased throughput, speed, sensitivity and flexibility, providing researchers with valuable insights at an unprecedented rate. While recent technological developments have made NGS more accessible to smaller labs, many barriers to implementation still remain. NGS workflows… Read article →


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Barcodes in NGS: sample vs molecular

NGS data can be extremely complex, and technology that helps improve the accuracy of data analysis can make the difference between drawing the right conclusions or the wrong ones. Sample and molecular barcodes serve roughly the same purpose as barcodes in any other context – they’re unique identifiers that distinguish and group like items together. But unless you’ve… Read article →


Biogenesis

miRNA-seq – when should you use it, and what are the challenges?

Exploring microRNA expression and function has led to some important discoveries about the regulation of gene expression in normal biological processes and disease states, and has even recently given rise to unique circulating biomarker signatures. qPCR and microarrays are the traditional techniques used to assess microRNA expression, and they have their strong points – qPCR… Read article →