Posts Tagged: NGS

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How pure is pure enough for DNA in NGS?

DNA purification is a critical step for obtaining accurate NGS data, because the quality of your starting material determines the reliability of next-generation sequencing results and data analysis. Even the best and most robust NGS technologies can only provide reliable results if the template nucleic acid is of adequate quality and purity. DNA purity is especially important at target… Read article →



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Getting started with NGS? Join our upcoming NGS series, beginning Sept. 8!

QIAGEN is hosting a four-part series on Tuesdays in September, “NGS overview, application, optimization and data analysis”, beginning September 8th: Part 1: Next-Generation Sequencing, an Introduction to Technology and Applications Sept 8, 1 pm EDT, 10 am PDT This webinar provides a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will cover… Read article →


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Next-generation sample preparation for liquid biopsy research

The use of next-generation sequencing (NGS) continues to grow due to several factors. Traditional Sanger sequencing performs a single read for each reaction, while NGS generates millions of sequencing reads simultaneously.  Although the accuracy of a single NGS sequence is lower, multiple reads are generated and aligned for a single run, generating an accurate consensus… Read article →


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Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →