Posts Tagged: NGS

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Next-generation sample preparation for liquid biopsy research

The use of next-generation sequencing (NGS) continues to grow due to several factors. Traditional Sanger sequencing performs a single read for each reaction, while NGS generates millions of sequencing reads simultaneously.  Although the accuracy of a single NGS sequence is lower, multiple reads are generated and aligned for a single run, generating an accurate consensus… Read article →


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Advances in sequencing single cells – a PCR-free method for NGS RNA library construction

In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →




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Environmental toxin biomarkers

What impact do environmental contaminants have on human health? Until recently, it was difficult to adequately address this complex question. From animal models and cell and tissue culture studies, it has been clear for quite some time that contaminants ranging from heavy metals to organic chemicals like BPA, PCBs and dioxins can be extremely detrimental… Read article →