Posts Tagged: PCR

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Quantitative readout of multiplex PCR assays using synthetic templates

Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →


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Getting Multiplex PCR gene expression profiling working for you

Multiplex PCR is an extremely enabling molecular biology technique, allowing the amplification of multiple targets in a single PCR experiment. There are a broad range of research applications for multiplex PCR, including pathogen identification, high-throughput SNP genotyping, gene expression profiling, template quantitation, RNA detection and even forensic studies. Gene expression profiling of CTCs  The presence… Read article →



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Top FAQs in qPCR

Since the development of high-throughput techniques such as next-generation sequencing and RNA-seq, “omics” type data has really taken off. Proven methods, like qPCR, are routinely used in combination with these high-throughput methods in labs across the world. Real-time PCR (quantitative PCR, qPCR) is a well-established method for gene expression analysis due to its superior sensitivity,… Read article →


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A 2-part webinar series: Advantages of multiplex PCR and automated PCR analysis with the QIAxcel Advanced

Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced. Multiplex PCR has a multitude of advantages and… Read article →