Posts Tagged: PCR

Epigenetics

Epigenetics research and its significance in cancer

Introduction to epigenetics and techniques There has been a great interest in the study of epigenetic mechanisms and DNA methylation in many areas of research, including DNA repair, cell cycle control, developmental biology, cancer research, identification of biomarkers, predisposition factors and potential drug targets. Lately I’ve been immersed in this field via my close work… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →


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Quantitative readout of multiplex PCR assays using synthetic templates

Have you ever thought about the precision required to get an accurate quantitative readout from a multiplex PCR assay? Multiplex PCR enables targeted, parallel amplification of multiple targets, and it comes with a significant set of challenges, including optimizing the PCR conditions to achieve precise, quantitative results across all target loci. All primers must function under the same reaction conditions, each primer… Read article →


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Dive into the QIAseq universe – introducing new QIAseq NGS portfolio

QIAGEN is making great strides towards improving your options in NGS library prep technology.  To explore the complete QIAseq NGS portfolio, download our most recent NGS launch flyer, “Exploring new frontiers with next-generation sequencing,” here! Here at QIAGEN, we take immense pride in developing innovative technologies and high-quality products to offer our customers a streamlined,… Read article →


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A winning combination in advanced genotyping

Fast and reliable multiplex PCR using high-quality gDNA Multiplex PCR technology is becoming standard in genotyping studies due to its revolutionary impact on experiments involving large numbers of PCR reactions, yielding major savings in both time and cost of reagents. Nowadays, genotyping and DNA testing applications in research, forensic and diagnostic laboratories all use multiplex PCR… Read article →