Posts Tagged: PCR


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Top FAQs in qPCR

Since the development of high-throughput techniques such as next-generation sequencing and RNA-seq, “omics” type data has really taken off. Proven methods, like qPCR, are routinely used in combination with these high-throughput methods in labs across the world. Real-time PCR (quantitative PCR, qPCR) is a well-established method for gene expression analysis due to its superior sensitivity,… Read article →


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A 2-part webinar series: Advantages of multiplex PCR and automated PCR analysis with the QIAxcel Advanced

Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced. Multiplex PCR has a multitude of advantages and… Read article →


Epigenetics

Epigenetics research and its significance in cancer

Introduction to epigenetics and techniques There has been a great interest in the study of epigenetic mechanisms and DNA methylation in many areas of research, including DNA repair, cell cycle control, developmental biology, cancer research, identification of biomarkers, predisposition factors and potential drug targets. Lately I’ve been immersed in this field via my close work… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →