Posts Tagged: PCR

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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →


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Dive into the QIAseq universe – introducing new QIAseq NGS portfolio

QIAGEN is making great strides towards improving your options in NGS library prep technology.  To explore the complete QIAseq NGS portfolio, download our most recent NGS launch flyer, “Exploring new frontiers with next-generation sequencing,” here! Here at QIAGEN, we take immense pride in developing innovative technologies and high-quality products to offer our customers a streamlined,… Read article →


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A winning combination in advanced genotyping

Fast and reliable multiplex PCR using high-quality gDNA Multiplex PCR technology is becoming standard in genotyping studies due to its revolutionary impact on experiments involving large numbers of PCR reactions, yielding major savings in both time and cost of reagents. Nowadays, genotyping and DNA testing applications in research, forensic and diagnostic laboratories all use multiplex PCR… Read article →


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Webinar takeaways – “Accurate DNA Methylation Analysis by Successful Bisulfite Conversion”

DNA methylation is widely recognized as a major player regulating normal gene expression and facilitating chromatin organization within cells. Research into abnormal DNA methylation that leads to human diseases like cancer could therefore provide valuable insights for disease development diagnosis and potential therapies. DNA is made up of 4 nucleotides – cytosine, guanine, thymine and… Read article →


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Top FAQs about digital RNAseq

Earlier this year, QIAGEN launched a new alternative for gene expression profiling which is based on digital RNA sequencing technology. It combines the power of NGS with the accuracy of qPCR and the analytical power of Ingenuity Pathway Analysis (IPA). As a part of this launch, we held a 3-part educational webinar series focused on… Read article →