Posts Tagged: qPCR


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Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →


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Keep calm, the chaperones are on!

The original nano-machines I was quite excited to hear the announcement that the 2016 Nobel Prize in Chemistry was awarded to a group of 3 European scientists for developing “nano-machines,” a technological breakthrough which crafted the path for the world’s first smart materials. Sir Fraser Stoddart, Bernard Feringa and Jean-Pierre Sauvage developed the world´s smallest… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →


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The PD1 race picks up pace

If you’ve been following developments in cancer immunotherapy closely, you’ve probably heard of programmed death 1 (PD1/PDCD1, also known as CD279) and PDL1 (CD274). Their interaction is at the center of new immune checkpoint-targeting therapies coming out of the pharmaceutical industry, such as Bristol-Myers Squibb’s nivolumab and Merck’s pembrolizumab (1, 5). What are PD1 and PDL1, and… Read article →