Posts Tagged: qPCR

Live qPCR webinar

Live webinar! The importance of controls and novel solutions for successful real-time qPCR

Reverse transcription quantitative PCR (RT-qPCR) is one of the most widely used methods for gene quantification today because it offers numerous advantages. High sensitivity, accuracy, speed and specificity are the key benefits that have made it a gold standard for gene expression analysis. However, a number of critical factors are involved during the various steps… Read article →



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Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →


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Keep calm, the chaperones are on!

The original nano-machines I was quite excited to hear the announcement that the 2016 Nobel Prize in Chemistry was awarded to a group of 3 European scientists for developing “nano-machines,” a technological breakthrough which crafted the path for the world’s first smart materials. Sir Fraser Stoddart, Bernard Feringa and Jean-Pierre Sauvage developed the world´s smallest… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →