Since the development of high-throughput techniques such as next-generation sequencing and RNA-seq, “omics” type data has really taken off. Proven methods, like qPCR, are routinely used in combination with these high-throughput methods in labs across the world. Real-time PCR (quantitative PCR, qPCR) is a well-established method for gene expression analysis due to its superior sensitivity,… Read article →
Time marches on and technology with it, and these new advances yield new perspectives. Of particular interest to this blog is the changing technology and perspective on microRNA profiling methods. Two years ago, I wrote about the pros and cons of 3 methods commonly used for miRNA expression analysis, NGS, qPCR and microarray, and concluded… Read article →
Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced. Multiplex PCR has a multitude of advantages and… Read article →
Cardiovascular diseases (CVD) are a leading cause of morbidity and mortality worldwide. Preventative and therapeutic strategies focused on controlling traditional risk factors have not always been successful. Recently, the use of miRNAs as novel biomarkers in CVD has opened up new possibilities for discovering novel therapeutic targets and understanding CVD development. To date, the total… Read article →
In recent years, research into the nervous system has advanced greatly, thanks to progress in areas like molecular biology, electrophysiology, biochemistry, high-resolution microscopy, computational neuroscience and nanotechnology. We can even understand some complex processes occurring in within a neuron. So nowadays, scientists can study several aspects of the nervous system including how it works, its… Read article →
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