Posts Tagged: qPCR


Biofluid microRNA profiling

The development of biomarkers will be essential for the future of diagnostic, prognostic and predictive medicine. Diagnostic biomarkers may be used to detect cancer, heart failure, stroke or a number of other diseases. Prognostic biomarkers predict patient outcome, while predictive biomarkers are essential for determining whether a cancer patient will respond to targeted therapy. Unfortunately, the translation… Read article →


Infographic: 5 tips for better qRT-PCR results

Market research shows that 66.6% of researchers use qRT-PCR for gene expression profiling. The popularity of this technique speaks to its effectiveness for detecting messenger RNA levels, but qRT-PCR can still be prone to pitfalls that sometimes lead to disappointing results. To help you improve your experiments, we’ve made a new infographic with interesting facts… Read article →


What enables drug resistance in ER+ breast cancer cells?

Chemoresistance is the bane of cancer therapy. Living things react and adapt to their environments; it would be convenient if cancer cells were the exception, but there are countless examples of a previously effective chemotherapy rendered impotent by a cancer’s adaptation. One of the major challenges in cancer research, then, is to decipher these adaptations… Read article →


A webinar with Dr. Jo Vandesompele – Expanding the dynamic range of microRNA expression

In 2014, an ambitious microRNA technology study was published in Nature Methods. This study evaluated the existing microRNA platforms on the market for their technical performance. The authors, including first author Pieter Mestdagh and corresponding author Jo Vandesompele of Ghent University and Biogazelle, found small differences between the qPCR technologies, but concluded that from this purely technical perspective, there was no… Read article →