Posts Tagged: qPCR

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What enables drug resistance in ER+ breast cancer cells?

Chemoresistance is the bane of cancer therapy. Living things react and adapt to their environments; it would be convenient if cancer cells were the exception, but there are countless examples of a previously effective chemotherapy rendered impotent by a cancer’s adaptation. One of the major challenges in cancer research, then, is to decipher these adaptations… Read article →



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A webinar with Dr. Jo Vandesompele – Expanding the dynamic range of microRNA expression

In 2014, an ambitious microRNA technology study was published in Nature Methods. This study evaluated the existing microRNA platforms on the market for their technical performance. The authors, including first author Pieter Mestdagh and corresponding author Jo Vandesompele of Ghent University and Biogazelle, found small differences between the qPCR technologies, but concluded that from this purely technical perspective, there was no… Read article →


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Why preamplify in miRNA research? Download the white paper

It’s hard to overstate the importance of amplification technology in molecular biology. Reverse transcription-polymerase chain reaction (RT-PCR) amplifies sections of messenger RNA so they can be quantified – we’d have a much dimmer view of the transcriptome without it. Whole-genome amplification helps us analyze genes in single or limited cells, opening up a whole new… Read article →


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Environmental toxin biomarkers

What impact do environmental contaminants have on human health? Until recently, it was difficult to adequately address this complex question. From animal models and cell and tissue culture studies, it has been clear for quite some time that contaminants ranging from heavy metals to organic chemicals like BPA, PCBs and dioxins can be extremely detrimental… Read article →