Posts Tagged: real-time PCR

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A 2-part webinar series: Advantages of multiplex PCR and automated PCR analysis with the QIAxcel Advanced

Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced. Multiplex PCR has a multitude of advantages and… Read article →



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Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →