Posts Tagged: real-time PCR


ILLU_0890_SingleCell_HeterogeneityTumorPeople (1)

Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →


S_5000_OneStep_iS18752900 (3)

8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →



miRNA_RISC_Ribosomes_700x233

Emerging roles of competing endogenous RNA (ceRNA)

If you have been following our blog posts about microRNA (miRNA) – or if you have been studying the microRNA world – you are likely familiar with the small 22-nucleotide RNAs that target messenger RNAs (mRNAs) to repress their translation into protein. The ceRNA hypothesis In 2011, Pier Paolo Pandolfi research lab proposed the hypothesis… Read article →