Posts Tagged: RNA



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Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →


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Barcodes in NGS: sample vs molecular

NGS data can be extremely complex, and technology that helps improve the accuracy of data analysis can make the difference between drawing the right conclusions or the wrong ones. Sample and molecular barcodes serve roughly the same purpose as barcodes in any other context – they’re unique identifiers that distinguish and group like items together. But unless you’ve… Read article →


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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →