Posts Tagged: RNA


Barcodes in NGS: sample vs molecular

NGS data can be extremely complex, and technology that helps improve the accuracy of data analysis can make the difference between drawing the right conclusions or the wrong ones. Sample and molecular barcodes serve roughly the same purpose as barcodes in any other context – they’re unique identifiers that distinguish and group like items together. But unless you’ve… Read article →

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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →

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The reproducibility crisis: what can we do?

There’s a growing trend of irreproducible results in biomedical research. In a previous post, Kurchi Bhattacharya reported on recent studies finding that a surprising number of scientists could not only not reproduce the results of their colleagues, but many found their own results difficult to reproduce as well. Much of the reproducibility problem likely has… Read article →

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One spin, one dedicated kit – selection wheels make it possible

It can be a real struggle sometimes to find and select the right isolation kit, whether it is for RNA or genomic DNA (gDNA) purification. Not using the right kit for your experiments has major consequences, however – the impact could be as severe as not being able to publish your results. Or worse, finding that other researchers were able… Read article →


Dive into the QIAseq universe – introducing new QIAseq NGS portfolio

QIAGEN is making great strides towards improving your options in NGS library prep technology.  To explore the complete QIAseq NGS portfolio, download our most recent NGS launch flyer, “Exploring new frontiers with next-generation sequencing,” here! Here at QIAGEN, we take immense pride in developing innovative technologies and high-quality products to offer our customers a streamlined,… Read article →