Posts Tagged: RT-qPCR

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Top FAQs in qPCR

Since the development of high-throughput techniques such as next-generation sequencing and RNA-seq, “omics” type data has really taken off. Proven methods, like qPCR, are routinely used in combination with these high-throughput methods in labs across the world. Real-time PCR (quantitative PCR, qPCR) is a well-established method for gene expression analysis due to its superior sensitivity,… Read article →


Biogenesis

miRNA-seq vs qPCR in 2017: an update

Time marches on and technology with it, and these new advances yield new perspectives. Of particular interest to this blog is the changing technology and perspective on microRNA profiling methods. Two years ago, I wrote about the pros and cons of 3 methods commonly used for miRNA expression analysis, NGS, qPCR and microarray, and concluded… Read article →


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A 2-part webinar series: Advantages of multiplex PCR and automated PCR analysis with the QIAxcel Advanced

Are you looking for a technology that combines specificity and sensitivity of parallel amplification reactions with high performance in a single reaction tube? In this 2-part webinar series, our experts will discuss how you can conduct successful multiplex PCR and automate your analysis with the QIAxcel Advanced. Multiplex PCR has a multitude of advantages and… Read article →



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Understanding tumor heterogeneity with gene expression analysis

Strategies to counter tumor heterogeneity are high on the wish list of oncologists and cancer researchers. Within a single tumor, cells can differ in size, proliferation rate and ability to metastasize. Most significantly for physicians and patients, they can also differ in their sensitivity to chemotherapy. How can we best understand and investigate this aspect… Read article →