Posts Tagged: RT-qPCR

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8 challenges to successful one-step RT-PCR

Once you’ve decided to do an RT-PCR experiment, you have a choice: one step or two? Both options have advantages and disadvantages, so it depends on your experimental goals. In 2-step RT-PCR, reverse transcription and PCR take place in different tubes, enabling the optimization of each separate reaction. This is useful if you have a… Read article →



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Emerging roles of competing endogenous RNA (ceRNA)

If you have been following our blog posts about microRNA (miRNA) – or if you have been studying the microRNA world – you are likely familiar with the small 22-nucleotide RNAs that target messenger RNAs (mRNAs) to repress their translation into protein. The ceRNA hypothesis In 2011, Pier Paolo Pandolfi research lab proposed the hypothesis… Read article →


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Cannabis epigenetic control of skin differentiation genes

Did you know that there is a “Hash, Marihuana & Hemp Museum” located in Amsterdam, Netherlands? Dedicated to education and scientific studies, this world-famous museum has received more than 2 million visitors since 1985. Despite its controversial uses and long banning histories, cannabis (commonly referred to as marijuana) and its chemical derivatives have been found… Read article →