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Expert talks how to purify more DNA from challenging tissues

Mechanical and enzymatic lysis both have their drawbacks when isolating genomic DNA from solid tissue samples. While mechanical disruption tends to provide high yields from tissue samples, it does damage the nucleic acids. Enzymatic lysis, like Proteinase K digestion, allows extraction of nucleic acids without damage, but requires long incubation times and provides lower yields… Read article →

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The reproducibility crisis: what can we do?

There’s a growing trend of irreproducible results in biomedical research. In a previous post, Kurchi Bhattacharya reported on recent studies finding that a surprising number of scientists could not only not reproduce the results of their colleagues, but many found their own results difficult to reproduce as well. Much of the reproducibility problem likely has… Read article →

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Still using xylene to manually extract DNA from your FFPE samples?

For decades, formalin-fixed paraffin-embedded (FFPE) tissue samples have been collected and archived for long-term storage. They’re extremely valuable for carrying out research like mutation screening or pathogen detection. In general, deparaffinization of the FFPE tissue sections is achieved by manual xylene pretreatment, which dissolves the paraffin from the tissue, and a series of ethanol washes… Read article →

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One spin, one dedicated kit – selection wheels make it possible

It can be a real struggle sometimes to find and select the right isolation kit, whether it is for RNA or genomic DNA (gDNA) purification. Not using the right kit for your experiments has major consequences, however – the impact could be as severe as not being able to publish your results. Or worse, finding that other researchers were able… Read article →

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How reliable are your samples?

Have you ever been in a situation where you prepared every necessary reagent for your experiment in the proper way, diligently performed every protocol step, and still ended up with background signals or even no signals in your gels, plots and blots? Have you conducted the experiment again while troubleshooting various components, but never found an answer? Wait – did you… Read article →

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