Posts Tagged: samples

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How reliable are your samples?

Have you ever been in a situation where you prepared every necessary reagent for your experiment in the proper way, diligently performed every protocol step, and still ended up with background signals or even no signals in your gels, plots and blots? Have you conducted the experiment again while troubleshooting various components, but never found an answer? Wait – did you… Read article →



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Phenol-based and non-phenol-based methods for extracting exosomal RNA

Studies in recent years have established that RNA contents in exosomes can serve as biomarkers for certain cancers (1). As exosomal RNA becomes more critical to research, successful isolation of these nanovesicles has become equally fundamental. Exosomes are small (40–100 nm) nanovesicles containing RNA that can move between cells. Found in several biological fluids, including blood plasma… Read article →


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Starting on the right foot – how to optimize RNA sample quality

RNA is one of the most important samples in molecular biology. In your lab’s personal biobank, you might extract RNA from any number of sample types, from frozen cells to FFPE tissue, and the insights you can gain from analyzing it are crucial. Messenger RNA links genes to proteins, and transcriptome profiling can reveal much about the workings… Read article →