Posts Tagged: samples

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Still using xylene to manually extract DNA from your FFPE samples?

For decades, formalin-fixed paraffin-embedded (FFPE) tissue samples have been collected and archived for long-term storage. They’re extremely valuable for carrying out research like mutation screening or pathogen detection. In general, deparaffinization of the FFPE tissue sections is achieved by manual xylene pretreatment, which dissolves the paraffin from the tissue, and a series of ethanol washes… Read article →


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One spin, one dedicated kit – selection wheels make it possible

It can be a real struggle sometimes to find and select the right isolation kit, whether it is for RNA or genomic DNA (gDNA) purification. Not using the right kit for your experiments has major consequences, however – the impact could be as severe as not being able to publish your results. Or worse, finding that other researchers were able… Read article →


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How reliable are your samples?

Have you ever been in a situation where you prepared every necessary reagent for your experiment in the proper way, diligently performed every protocol step, and still ended up with background signals or even no signals in your gels, plots and blots? Have you conducted the experiment again while troubleshooting various components, but never found an answer? Wait – did you… Read article →



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Phenol-based and non-phenol-based methods for extracting exosomal RNA

Studies in recent years have established that RNA contents in exosomes can serve as biomarkers for certain cancers (1). As exosomal RNA becomes more critical to research, successful isolation of these nanovesicles has become equally fundamental. Exosomes are small (40–100 nm) nanovesicles containing RNA that can move between cells. Found in several biological fluids, including blood plasma… Read article →