Posts Tagged: samples


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Understanding what factors contribute to variations in quantity of DNA purified from FFPE materials

QIAGEN recently presented a webinar exploring what factors contribute to variations in quantity of DNA purified from FFPE material. As noted in “Top 5 considerations for preparing and archiving FFPE samples,” FFPE is a standard method for long-term preservation of DNA in tissue biopsies. It’s critical to understand how to overcome the challenges that this sample type presents in order… Read article →


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How pure is pure enough for DNA in NGS?

DNA purification is a critical step for obtaining accurate NGS data, because the quality of your starting material determines the reliability of next-generation sequencing results and data analysis. Even the best and most robust NGS technologies can only provide reliable results if the template nucleic acid is of adequate quality and purity. DNA purity is especially important at target… Read article →


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Cryopreservation as a method for long-term sample storage

Cryopreservation (cryogenic preservation) is “the storage of cells, tissues and organs following appropriate ‘preparatory’ procedures at the ultra-low temperature of liquid nitrogen (–196°C).” (1) This form of preservation is ideal for biobanking because these cold temperatures slow chemical and physical reactions of the biomolecules and biospecimens and stop the samples from degrading. Liquid nitrogen biobanks… Read article →


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The 12 SOPs every biobank should have

High-quality biobanked samples closely resemble the biospecimen prior to extraction, with minimal modification due to collection or storage conditions. Establishment of standard operating procedures (SOPs) is crucial to avoid introducing variables into your research samples during storage and maintain the high quality required for reliable results. According to current recommendations, there are 12 SOPs that… Read article →