Whether you’re studying single cell genomics, circulating tumor cells or the microbiome, analyzing single cells creates the potential for a deeper understanding of cell heterogeneity. But several challenges stand in the way of this goal, such as allelic dropout, coverage uniformity and low yield. How can you overcome these? The single cell team at QIAGEN… Read article →
Posts Tagged: single cell
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In our last post, “How can you construct NGS DNA libraries from a single cell in just one day?”, we discussed how to prepare a DNA NGS library from a single cell. What are the differences when preparing libraries for RNA-seq? We know that a number of conventional whole transcriptome amplification methods already overcome limited RNA availability,… Read article →
Are you working with single cells or a very low number of cells and want to analyze by next-generation sequencing? Well, we do too. However, challenges like small sample amounts and the heterogeneity of the input materials make designing the right method critical to effective analysis. What’s the best way to conduct genomic and transcriptomic… Read article →
Whole genome sequencing starting from one individual cell requires a genomic DNA amplification step before the NGS library preparation. Even technologies like multiple displacement amplification (MDA), proven to be superior in genome coverage (2) and fidelity (3), are not completely bias-free. Uneven genomic coverage, in particular, directly impacts the sensitivity of variant detection in WGA-amplified single… Read article →